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KMID : 0356620090240010017
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Journal of Korean Society of Endocrinology
2009 Volume.24 No. 1 p.17 ~ p.24
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Expression of miRNA 146a/b, 221 and 222 in Thyroid Cancer
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Jo Young-Suk
Lee Ihn-Suk
Hong Woo-Jeong
Song In-Sang
Song Min-Ho
Kim Je-Ryoung
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Abstract
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Background: miRNAs can be diagnostic markers and therapeutic targets in cancers, but few studies have been conducted in thyroid cancer. We investigated the expression levels of miRNA 146a/b, 221, and 222 which are important miRNAs in papillary thyroid cancers (PTCa), and verified their impact on clinicopathological factors.
Methods: We measured the expression of pre-miRNAs 146a/b, 221, and 222 in NPA cells treated with 10%
fetal bovine serum (FBS) or in HEK293T cells transfected with RET/PTC3 or BRAFV600E expression vectors.
We also investigated the relationship between miRNA expression levels in thyroid cancer tissue specimens and
clinicopathological parameters.
Results: Growth stimulation with 10% FBS induced miRNA expressions in NPA cells, and transfection of
RET/PTC3 and BRAFV600E also increased the expression of these miRNAs in HEK293T cells. Most (25 cases; 50%) of PTCa showed increased expression of miRNA-146a/b and 30 cases (60%) had elevated expression of miRNA-221 and miRNA-222 compared to normal thyroid samples from the contralateral lobe. However,
increased miRNA expression did not correlate with clinicopathological factors.
Conclusions: Expression of miRNA 146a/b, 221, and 222 was increased by BRAFV600E and RET/PTC3
rearrangement and might have a role in tumorigenesis in PTCa. However, expression levels of these miRNAs did not correlate with clinicopathological parameters of patients with PTCa.
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KEYWORD
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microRNA, prognosis, thyroid neoplasm
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